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For transfection, the cells were cultured until 70% confluency and transfected with the pCMV-2B FLAG-GST expression vector using Lipofectamine 3000 reagent in Opti-MEM (Gibco) medium according to the manufacturers protocol (Invitrogen)

You can enjoy a healthier complexion without any concerns

A lack of cysteine and methionine can cause a lack of glutathione, allowing hydrogen peroxide molecules to accumulate

How are proteins reduced in the endoplasmic reticulum

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